Thermo Fisher Scientific TSC1 Monoclonal Antibody (OTI5A8), TrueMAB

Applications and Tested Dilutions

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882) before use.

Target Information

Tuberous sclerosis complex (TSC) is an autosomal dominant tumor syndrome caused by mutations in either the TSC1 or TSC2 tumor suppressor genes. The protein products of these genes form a complex that indirectly decreases the signaling of the mammalian Target of Rapamycin (TOR), a conserved serine/threonine kinase regulating cell growth and cycle control.

TOR activity is stimulated by Rheb, a Ras superfamily G-protein, when the GTP/GDP ratio is high. Immunoprecipitated TSC1/TSC2 complex stimulates Rheb GTPase activity, thereby reducing TOR activation. Overexpression of TSC1 and TSC2 decreases Rheb GTP/GDP ratio and inhibits cell growth.
A shorter 40 kDa isoform of TSC1 has been observed, though its function remains unknown.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.