Thermo Fisher Scientific CD4 Monoclonal Antibody (S3.5), Pacific Orange

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Assay-Dependent

View 3 publications 3 publications

Miscellaneous PubMed (Misc)

-

View 17 publications 17 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Non-human primate, Rhesus monkey

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

S3.5

Immunogen

Human CD4

Conjugate

Pacific Orange™ Pacific Orange™ Pacific Orange™

View additional formats

• Unconjugated
• Alexa Fluor 405
• Alexa Fluor 488
• Alexa Fluor 700
• APC
• APC-Alexa Fluor 750
• APC-Cyanine5.5
• FITC
• Pacific Blue
• PE
• PE-Alexa Fluor 610
• PE-Alexa Fluor 700
• PE-Cyanine5.5
• PE-Cyanine7
• PE-Texas Red
• PerCP
• Qdot 605
• Qdot 655
• Qdot 705
• Qdot 800
• TRI-COLOR
• Request custom conjugation

Excitation/Emission Max

400/551 nm View spectra

Form

Liquid

Purification

purified

Storage buffer

PBS with BSA

Contains

0.1% sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10371907

Product Specific Information

Our Pacific Orange™ dye is optimally excited by the violet laser, and it is recommended that a 575, 585 or 600 nm band pass filter be used for optimal detection. Pacific Blue™ and Pacific Orange™ dye conjugates can be simultaneously excited at 405 nm and emit at 455 nm and 551 nm, respectively, facilitating two-color analysis.

Target Information

The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].

Analyte Specific Reagent