Thermo Fisher Scientific ALAS1 Monoclonal Antibody (OTI5D5), TrueMAB

Applications

• Western Blot (WB)
  Tested Dilution: 1:2,000

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

ALAS1 is a nuclear-encoded mitochondrial matrix enzyme that catalyzes the condensation of glycine with succinyl-CoA to form δ-aminolevulinate, CO₂, and CoA.
It regulates the first and rate-limiting step of the heme biosynthetic pathway and serves as a pyridoxal-5′-phosphate-dependent housekeeping enzyme.
Ubiquitously expressed, ALAS1 provides heme for cytochromes and other hemoproteins.
Its expression in the liver is negatively regulated by heme and modulated by cAMP, respiratory uncoupling, phorbol esters, and insulin.
ALAS1 may play a role in the reciprocal regulation of heme biosynthesis and the circadian clock, suggesting potential therapeutic relevance for circadian disorders.
Downregulation of ALAS1 expression is associated with acute liver failure and altered heme metabolism.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.