Thermo Fisher Scientific CD8a Monoclonal Antibody (RPA-T8), Brilliant Violet 650, eBioscience

Thermo Fisher Scientific CD8a Monoclonal Antibody (RPA-T8), Brilliant Violet 650, eBioscience™

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.5 µg)/test

Product Specifications

Available Conjugate Formats

• Alexa Fluor 532, 561, 660, 700
• APC, APC-eFluor 780
• Brilliant UV Series (395~805)
• Brilliant Violet Series (421~786)
• eFluor 450, 506
• FITC, PE, PE-Cyanine Series
• PerCP-Cyanine5.5, Super Bright Series
• NovaFluor Series
• Request custom conjugation

Product Specific Information

The RPA-T8 monoclonal antibody recognizes the human CD8a molecule (32–34 kDa), expressed as CD8αβ heterodimers or CD8αα homodimers. CD8a is found on thymocytes, mature T cells, and NK cells, and binds MHC class I molecules, playing a role in T-cell development and activation.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-diluted and validated for flow cytometry using normal human peripheral blood cells at 5 µL/test (0.5 µg/test).
• Fluorochrome: Brilliant Violet™ 650 (BV650), emission at 649 nm, suitable for violet (405 nm) laser.
• Buffer Recommendation: Use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize polymer dye interactions.
• Light Sensitivity: Protect from light to avoid photo-induced oxidation.
• Fixation Compatibility: Compatible with IC Fixation Buffer (00-8222-49) or 1-step Fix/Lyse Solution (00-5333-54); not compatible with methanol-based fixation.
• Excitation / Emission: 407 nm / 649 nm (Violet Laser)

Trademark Notice: BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

CD8 (Cluster of Differentiation 8) is a type I transmembrane glycoprotein of the immunoglobulin receptor family, essential for signal transduction, T-cell differentiation, and activation.
It functions as a co-receptor with TCR for MHC class I molecules, while CD4 interacts with MHC class II.
CD8 is expressed on T cells, NK cells, monocytes, and dendritic cells, existing as CD8αβ heterodimers or CD8αα homodimers.
Engagement with MHC-I/peptide complexes recruits Lck kinase, leading to cytokine production and cytotoxic T lymphocyte activation.
Differentiation of naive CD8+ T cells into CTLs is enhanced by IL-2, IL-12, and TGF-β1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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