Thermo Fisher Scientific CD8a Monoclonal Antibody (53-6.7), Brilliant Violet 650, eBioscience

Thermo Fisher Scientific CD8a Monoclonal Antibody (53-6.7), Brilliant Violet 650, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.5 µg/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 561, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 및 Violet 시리즈 (395~805)
• eFluor 시리즈 (450~660)
• FITC
• Functional Grade
• NovaFluor 시리즈 (Blue, Red, Violet, Yellow)
• PE, PE-Cyanine5, 5.5, 7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 시리즈 (436~780)
• Request custom conjugation

Product Specific Information

The 53-6.7 monoclonal antibody reacts with the mouse CD8a molecule, a 32–34 kDa surface receptor expressed as CD8αβ heterodimers or CD8αα homodimers.
CD8 interacts with MHC class I and associates with tyrosine kinase p56lck, playing a role in T cell development and activation.

Applications Reported: Flow cytometric analysis.
Applications Tested: Mouse splenocytes, ≤0.5 µg/test in 100 µL volume. Optimal titration recommended.

Brilliant Violet™ 650 (BV650):

• Tandem dye emitting at 649 nm, excitable by 405 nm violet laser.
• Use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize polymer interactions.
• Light-sensitive; protect from photo-induced oxidation.
• Fixation compatible with IC Fixation Buffer (00-8222-49) or 1-step Fix/Lyse Solution (00-5333-54) for up to 3 days at 4°C.
• Not compatible with methanol-based fixation.

Excitation: 407 nm
Emission: 649 nm
Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

CD8 is a type I transmembrane glycoprotein involved in signal transduction and T cell activation.
It acts as a co-receptor for MHC class I molecules, primarily expressed on T cells, NK cells, monocytes, and dendritic cells.
Upon MHC-I/peptide complex binding, CD8 recruits Lck kinase, triggering CTL activation and pathogen/tumor clearance.
Differentiation of naive CD8+ T cells into cytotoxic T lymphocytes is enhanced by IL-2, IL-12, and TGF-β1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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