Thermo Fisher Scientific LAG3 Recombinant Rabbit Monoclonal Antibody (BLR027F)
LAG3 단백질을 인식하는 토끼 단클론 항체로, Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 적합합니다. HEK293 세포에서 발현된 재조합 항체이며, 높은 특이성과 재현성을 제공합니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| Flow Cytometry (Flow) | 1 µL / 1 × 10⁶ cells |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | BLR027F |
| Immunogen | Peptide representing a region between residues 500–525 of human lymphocyte activation gene 3 protein (LAG3) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.50 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | BBS, pH 8.2, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt.
- For ICC: Epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE cell sections.
- For IHC: Epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections.
Target Information
LAG-3 (Lymphocyte Activation Gene 3) is a 70-kDa surface glycoprotein belonging to the immunoglobulin superfamily, showing homology to CD4. It binds to MHC class II with higher affinity than CD4 and is involved in negative regulation of T cell activation and homeostatic proliferation. LAG-3 expression is observed on activated T cells (including regulatory T cells) and NK cells, with CD8⁺ T cells generally expressing higher levels than CD4⁺ T cells. Coexpression of LAG-3 and CD49b has been proposed as a marker for Type 1 regulatory T cells (Tr1 cells) in humans and mice.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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