Thermo Fisher Scientific CD218a (IL-18Ra) Monoclonal Antibody (P3TUNYA), PE-Cyanine7, eBioscience

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.5 µg/test

View 3 publications 3 publications

Product Specifications

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE-Cyanine7, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

P3TUNYA

Conjugate

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

View additional formats

• APC
• eFluor 450
• PE
• PerCP-eFluor 710
• Request custom conjugation

Excitation/Emission Max

569/780 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Wet ice

RRID

AB_2762704

Product Specific Information

Description: This P3TUNYA monoclonal antibody reacts with mouse CD218a (Interleukin 18 Receptor alpha). CD218a is one of the subunits of the heterodimeric receptor complex for the IL-18 cytokine. IL-18 is a proinflammatory cytokine and member of the IL-1 family first identified by its role in IFN gamma secretion during toxic shock. Like the other members of the family, it is synthesized as an inactive precursor which requires cleavage by caspases prior to secretion of the active form. It is secreted by activated monocytes, macrophages, epithelial cells, and keratinocytes via a non-classical pathway.

IL-18 Receptor is expressed on Th1, but not naive CD4 T cells or Th2, as well as subsets of NK cells and neutrophils. The heterodimeric receptor is made up of a ligand-binding alpha chain and signal-transducing beta chain. On its own, IL-18Ra binds IL-18 with low affinity, although the affinity of this interaction increases upon forming a complex with IL-18Rb. IL-18R signaling plays a critical role in Th1 differentiation and effector function, and results in the increased expression of IFN gamma, TNF alpha, and Fas ligand from T cells and NK cells. IL-18 has also been found to synergize with IL-23 in the development and function of Th17 cells. Recently, a subset of CD8 T cells has been discovered that co-secretes IFN gamma with IL-17A and sometimes IL-22. These Tc17 cells share key transcription factors, chemokine receptors, and cytokine receptors with Th17 cells. In humans, this population has been identified by expression of CD161 and IL-18R. As a CD161 homolog has yet to be identified in mice, it has been suggested that IL-18R may be used as a surrogate for CD161 in mouse Tc17 phenotyping.

Applications Reported: This P3TUNYA antibody has been reported for use in flow cytometric analysis.

Applications Tested: This P3TUNYA antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Target Information

IL18R1 is a cytokine receptor that belongs to the interleukin 1 receptor family. This receptor specifically binds interleukin 18 (IL18), and is essential for IL18 mediated signal transduction. IFN-alpha and IL12 are reported to induce the expression of this receptor in NK and T cells. The gene along with four other members of the interleukin 1 receptor family, including IL1R2, IL1R1, ILRL2 (IL-1Rrp2), and IL1RL1 (T1/ST2), form a gene cluster on chromosome 2q.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.