Thermo Fisher Scientific CD85d (ILT4) Monoclonal Antibody (42D1), PE-Cyanine7, eBioscience

Thermo Fisher Scientific CD85d (ILT4) Monoclonal Antibody (42D1), PE-Cyanine7, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.5 µg)/test

Product Specifications

Additional Formats

• APC
• Functional Grade
• NovaFluor Red 710
• PE
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The monoclonal antibody 42D1 recognizes the CD85d protein (also known as ILT4, LIR-2). It is a member of the ILT/LIR/MIR receptor family. CD85d is a 110 kDa single transmembrane receptor with 3 Ig-like domains and a long cytoplasmic domain containing ITIMs (immunoreceptor tyrosine-based inhibitory motifs). Expression is confined to monocytes, macrophages, and dendritic cells. The ligand for CD85d is non-classical class I HLA-G, -B, and G1. Upon ligand binding, tyrosine phosphatase SHP-1 interacts with the ITIM. Interaction of HLA-G with ILT4/CD85d contributes to tolerance during fetal implantation and may play a role in organ allograft rejection.

Crosslinking of the receptor with the monoclonal antibody 42D1 inhibits calcium flux, enhances HLA-G tetramer staining, and restores the stimulating activity of tolerogenic dendritic cells.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and tested on normal human peripheral blood cells. Use 5 µL (0.5 µg) per test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test.

Light Sensitivity: This tandem dye is sensitive to photo-induced oxidation. Protect vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency.

Excitation: 488–561 nm
Emission: 775 nm
Laser: Blue, Green, Yellow-Green

Target Information

Gene: LILRB2 (ILT4)
Located at chromosomal region 19q13.4, LILRB2 is a member of the leukocyte immunoglobulin-like receptor (LIR) family. It belongs to the subfamily B class of LIR receptors containing two or four extracellular Ig domains, a transmembrane domain, and two to four cytoplasmic ITIMs. Expressed on immune cells, it binds MHC class I molecules and transduces inhibitory signals to control inflammatory responses and cytotoxicity, helping limit autoreactivity. Multiple transcript variants encoding different isoforms exist.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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