Thermo Fisher Scientific Human IL-17A Recombinant Protein
인간 유래 IL-17A 재조합 단백질로 염증 반응 연구에 사용됩니다. E. coli 발현 시스템에서 제조되었으며, >95% 순도와 낮은 엔도톡신 수준을 갖습니다. 다양한 실험(ELISA, WB, IHC 등)에 활용 가능하며, 동결건조 형태로 안정적 보관이 가능합니다.
- 카탈로그번호
- PHC917x (3개 옵션)
- 카테고리
- Protein
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Human IL-17A Recombinant Protein
Applications
- Western Blot (WB): Assay-dependent
- Immunohistochemistry (IHC): Assay-dependent
- ELISA: Assay-dependent
- Functional Assay: Assay-dependent
- Control (Ctrl): Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Human |
| Expression System | E. coli |
| Amino Acid Sequence | Human IL-17A recombinant protein is homodimeric containing two 137 amino acid subunits |
| Molecular Weight | 15.5 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity | >95% by SDS-PAGE |
| Endotoxin Concentration | <0.1 ng/µg |
| Activity | ED50 = 0.5 - 5.0 ng/mL; determined by the dose-dependent production of GRO-alpha by HT-29 cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Purified |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice (domestic); Wet or Dry ice (international) |
Product Specific Information
Carrier-Free
Reconstitution:
We recommend that the vial be briefly centrifuged prior to opening to bring the contents to the bottom.
Lyophilized human IL-17A should be reconstituted in sterile, distilled water to a concentration of 0.1–1.0 mg/mL.
For maximal stability, a carrier protein such as 0.1–1.0% BSA may be included.
The reconstituted protein should be apportioned into working aliquots and stored at ≤ -20°C.
Further dilutions should be made in low endotoxin medium or buffered solution with FBS or tissue culture grade BSA.
Storage:
For maximal stability, store this lyophilized preparation at ≤ -20°C, preferably desiccated.
For shipment and short-term storage (up to 2 weeks), storage at 2–8°C is sufficient.
Reconstituted human IL-17A should be stored at ≤ -20°C (not in a frost-free freezer).
Keep freeze-thaw cycles to a minimum.
Target Information
Interleukin-17A (IL-17A, CTLA-8) is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection.
IL-17A is a 32 kDa, disulfide-linked homodimer consisting of 136 amino acids, a member of a six-protein family (IL-17A–17F), signaling through the IL-17 receptor (IL-17R/CDw217).
High levels of IL-17A homodimer are produced by activated peripheral blood CD4+ T-cells.
IL-17A enhances expression of ICAM-1 in human fibroblasts and stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2.
In the presence of IL-17A, fibroblasts can sustain proliferation of CD34+ hematopoietic progenitors and induce maturation into neutrophils.
Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating cross-species receptor recognition.
IL-17A regulates NF-kappa B and MAP kinase activities, stimulates IL-6 and COX-2 expression, and enhances nitric oxide (NO) production.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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