Thermo Fisher Scientific Human Dkk-1 Recombinant Protein

Applications

Western Blot (WB)

• Assay-dependent

Immunohistochemistry (IHC)

• Assay-dependent

ELISA

• Assay-dependent

Functional Assay

• Assay-dependent

Control

• Assay-dependent

Product Specifications

Product Specific Information

Carrier-Free

• Reconstitution:
  Centrifuge the vial briefly before opening to bring contents to the bottom. Reconstitute the lyophilized protein in sterile, distilled water to a concentration of 0.1–0.5 mg/mL. Apportion the reconstituted protein into working aliquots and store at ≤ -20°C. Further dilutions should be made in low endotoxin medium or buffered solution containing carrier protein such as heat-inactivated FCS or tissue culture grade BSA.

• Storage:
  Store the lyophilized protein at 2–8°C or -20°C for long term, preferably desiccated. Upon reconstitution, apportion into working aliquots and store at ≤ -20°C. Avoid repeated freeze-thaw cycles.

Target Information

DKK-1 is a member of the DKK protein family (DKK-1, DKK-2, DKK-3, DKK-4). It was originally identified as a Xenopus head-forming molecule acting as an antagonist for Wnt signaling. DKK-1 and DKK-4 regulate the Wnt/β-catenin signaling pathway by forming inhibitory complexes with LDL receptor-related proteins 5 and 6 (LRP5 and LRP6), essential components of the Wnt signaling system. These single-pass transmembrane proteins act as co-receptors for Wnt ligands. Inhibition of Wnt/β-catenin signaling by DKK-1 enables anterior development, as evidenced by DKK-1-deficient mice lacking head formation and dying during embryogenesis.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.