Thermo Fisher Scientific P2X7 Receptor (extracellular) Polyclonal Antibody
P2X7 수용체의 세포외 영역을 인식하는 Rabbit Polyclonal 항체로, WB, IHC(F), ICC/IF, Flow Cytometry 등에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, Lyophilized 형태로 제공되어 안정적 보관이 가능합니다. 연구용으로만 사용됩니다.
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Thermo Fisher Scientific P2X7 Receptor (extracellular) Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 2.5 µg |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse, Rat, Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KKGWMDPQSKGIQTGRC (amino acid residues 136–152 of mouse P2X7 receptor, extracellular loop) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.8 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Reconstitution can be performed with 25 µL, 50 µL, or 0.2 mL of double distilled water (DDW), depending on sample size.
The antibody is shipped lyophilized at room temperature and should be stored at -20°C upon arrival.
Reconstituted solution can be stored at 4°C for up to 1 week; for longer storage, aliquot and keep at -20°C.
Avoid repeated freeze/thaw cycles.
Centrifuge all antibody preparations before use (10,000 × g for 5 min).
Target Information
The P2X7 receptor is a member of the purinoceptor family for ATP, functioning as a ligand-gated ion channel. It mediates ATP-dependent lysis of macrophages via formation of membrane pores permeable to large molecules. Activation by ATP in the cytoplasm may link cellular activity to changes in gene expression.
Multiple alternatively spliced variants have been identified, most of which meet nonsense-mediated decay criteria.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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