
Norgen UltraRanger 1 kb DNA Ladder
고분자량 DNA 절편 크기 측정에 최적화된 1 kb DNA Ladder. 15개의 명확한 밴드(300~24000 bp)로 구성되어 있으며, 5000 bp와 10000 bp에서 참조 밴드 강화. 사용 준비 완료 상태로 정량적이고 안정적. 최대 100회 사용 가능.
- 카탈로그번호
- 12100
- 브랜드
- Norgen
- 카테고리
- Other Organics
- 판매단위
- pk
카탈로그
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Norgen UltraRanger 1 kb DNA Ladder
SKU: 12100
Ideal for sizing digested DNA of high molecular weight.
Features and Benefits
- Ready-to-use
- Quantitative
- Highly stable
- Precise
- Fifteen discrete fragments ranging from 300 bp to 24000 bp
- Higher intensity reference bands at 5000 bp and 10000 bp
The Norgen UltraRanger 1 kb DNA Ladder is prepared to ensure quality and batch-to-batch consistency. It contains fifteen discrete fragments ranging from 300 bp to 24000 bp with higher intensity reference bands at 5000 bp and 10000 bp. This ladder is ideal for sizing digested DNA of high molecular weight.
Contents
1 mL of premixed DNA ladder (0.5 µg/10 µL) in loading buffer (10 mM EDTA, 10% glycerol, 0.015% bromophenol blue, 0.17% SDS).
Ladder Properties
UltraRanger 1 kb DNA Ladder (Cat# 12100) – 100 loads
| Fragment | Size (bp) | Mass (ng) |
|---|---|---|
| 1 | 24000 | 30 |
| 2 | 14000 | 27 |
| 3 | 10000 | 60 |
| 4 | 8000 | 44 |
| 5 | 6000 | 36 |
| 6 | 5000 | 55 |
| 7 | 4000 | 49 |
| 8 | 3000 | 39 |
| 9 | 2500 | 35 |
| 10 | 2000 | 30 |
| 11 | 1500 | 33 |
| 12 | 1000 | 10 |
| 13 | 700 | 21 |
| 14 | 500 | 18 |
| 15 | 300 | 11 |
Recommended Use
Mix thoroughly before use. For best results, load 10 µL of DNA ladder per well.
For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5 µg/mL ethidium bromide for 30–40 minutes.
The table above shows the size and mass for each band based on 10 µL ladder per well.
Storage
Stable at room temperature. For long-term storage, −20 °C is recommended.
Standardized using 10 µL of DNA per lane on a 0.8 cm thick, 13 × 15 cm, 1.0% agarose gel run in TAE buffer.
Supporting Data (OCR 추출 내용)
UltraRanger 1 kb DNA Ladder Band Information
| bp | ng/10 µL |
|---|---|
| 24000 | 30 |
| 14000 | 27 |
| 10000 | 60 |
| 8000 | 44 |
| 6000 | 36 |
| 5000 | 55 |
| 4000 | 49 |
| 3000 | 39 |
| 2500 | 35 |
| 2000 | 30 |
| 1500 | 33 |
| 1000 | 10 |
| 700 | 21 |
| 500 | 18 |
| 300 | 11 |
Note: 10 µL loaded on 0.5% agarose gel.
Documentation
- 12100 – Product Insert (PDF)
- DNA Quantification by Gel Densitometry with Norgen DNA Ladders (App Note)
- UltraRanger 1 KB DNA Ladder – SDS
Citations
| Title | Journal | Authors |
|---|---|---|
| The genome and proteome of Serratia bacteriophage eta which forms unstable lysogens. | Virology Journal, 2014 | Denyes JM et al. |
| Red/ET recombination with chimeric oligonucleotides allows rapid generation of BAC transgenes harboring full-length or truncated huntingtin cDNA. | Biotechniques, 2012 | Hager S et al. |
| Differences in the quantity of DNA found in the urine and saliva of smokers versus nonsmokers: implications for the timing of epigenetic events. | Epigenomics, 2012 | Simkin M et al. |
| Variation of Serine-Aspartate Repeats in Membrane Proteins Possibly Contributes to Staphylococcal Microevolution. | PLOS One, 2011 | Cheng J et al. |
| Unique Changes in Mitochondrial Genomes Associated with Reversions of S-Type Cytoplasmic Male Sterility in Maize. | PLoS ONE, 2011 | Matera J et al. |
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