Thermo Fisher Scientific Phospho-FAK (Tyr861) Polyclonal Antibody
FAK 단백질의 Tyr861 인산화 부위를 특이적으로 인식하는 Rabbit Polyclonal Antibody. Western blot, IHC, ICC 등 다양한 응용에 적합하며, BSA 포함 PBS buffer에 보관. Integrin 신호전달 및 세포 부착 연구에 유용.
- 카탈로그번호
- 44626G
- 판매단위
- pk
카탈로그
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 35 publications |
| Immunohistochemistry (IHC) | Assay-dependent | View 13 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | View 5 publications |
| Immunocytochemistry (ICC/IF) | 1:250 | View 11 publications |
| Immunoprecipitation (IP) | - | View 2 publications |
| Miscellaneous PubMed (Misc) | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Chicken, Human, Mouse |
| Published Species | Chicken, Dog, Hamster, Human, Mouse, Non-human primate, Rat, Xenopus, Zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Chemically synthesized phosphopeptide derived from human FAK containing tyrosine 861. Sequence conserved in mouse, rat, chicken, and frog. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification | Affinity chromatography |
| Storage Buffer | Dulbecco’s PBS (pH 7.3) with 1 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2533703 |
Product Specific Information
- Antibody preadsorbed with non-phosphopeptide to remove cross-reactivity with non-phosphorylated FAK.
- Final product purified using FAK-derived peptide phosphorylated at Tyr861.
- Positive controls: Primary chicken embryo fibroblasts (CEF) expressing FAK and A459 whole cell lysate.
- Target antigen MW (Western blot): 125 kDa.
Target Information
Focal Adhesion Kinase (FAK) is a 125 kDa non-receptor protein tyrosine kinase acting as a substrate for Src and playing a key role in integrin signaling.
FAK regulates cell spreading, differentiation, migration, apoptosis, and cell cycle progression (G1 to S phase).
It localizes to focal adhesions via its C-terminal tail and mediates interactions with extracellular matrix through integrin receptors.
Phosphorylation of FAK tyrosines increases upon integrin engagement with fibronectin, promoting association with Grb2 and c-Src and activation of ERK2 MAP kinase.
FAK gene has several transcript variants encoding different isoforms, with three full-length forms characterized.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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