Thermo Fisher Scientific Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Biotin
염소 유래의 항-토끼 IgG(H+L) 2차 항체로, Biotin으로 결합되어 다양한 검출 시스템에 활용 가능. Western blot, ICC/IF, Flow cytometry, ELISA에 적합. 고도로 교차흡수되어 비특이적 결합 최소화. 연구용으로 높은 민감도와 재현성 제공.
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Applications and Tested Dilution
| Application | Dilution Range |
|---|---|
| Western Blot (WB) | 1:10,000–1:50,000 |
| Immunocytochemistry (ICC/IF) | 1:500–1:20,000 |
| Flow Cytometry (Flow) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Rabbit |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobulins Heavy and Light chains |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C (short term). For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2534787 |
| Target | IgG |
| Cross Adsorption | Against bovine, goat, human, mouse, and rat IgG and serum |
| Antibody Form | Whole Antibody |
Additional Formats Available
- Alexa Fluor 350, 488, 546, 555, 568, 594, 633, 635, 647, 660, 680, 790
- Alexa Fluor Plus 405, 488, 555, 594, 647, 680, 800
- AP, FITC, HRP, Oregon Green 488, TRITC
- Custom conjugation available upon request
Target Information
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins. These antibodies are useful in detection, sorting, or purification of rabbit IgG targets. Secondary antibodies enhance versatility and sensitivity by allowing the use of multiple detection systems (e.g., HRP, AP, fluorescence) and signal amplification. Typically generated by immunizing the host animal with pooled immunoglobulins from the target species, they may undergo purification or modification (e.g., immunoaffinity chromatography, fragmentation, label conjugation) to produce highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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