Thermo Fisher Scientific Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, TRITC

Thermo Fisher Scientific Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, TRITC

Applications and Tested Dilution

• Immunocytochemistry (ICC/IF): 4 µg/mL

Product Specifications

Product Specific Information

Rehydrate with 1.1 mL of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.)
Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Shelf life: 1 year from date of receipt.
Prepare working dilution prior to use and then discard.
Based on immunoelectrophoresis, no reactivity is observed to non-immunoglobulin rabbit serum proteins, serum proteins from bovine, human, or mouse, IgG from human or mouse.

Target Information

Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting, or purification of its specified target.
Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence).
They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody.
Most commonly, secondary antibodies are generated by immunizing the host animal with pooled immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Available Conjugate Formats

• Alexa Fluor 350, 405, 430, 488, 514, 532, 546, 555, 568, 594, 633, 635, 647, 660, 680, 700, 750, 790
• AP, APC, Biotin, Biotin-XX, Cyanine3, Cyanine5
• DyLight 350, 405, 488, 550, 594, 633, 650, 680, 755, 800
• FITC, HRP, Pacific Blue, Pacific Orange, PE, PE-Alexa Fluor 610, PE-Alexa Fluor 647
• Rhodamine, Rhodamine Red-X, Texas Red, Texas Red-X
• Custom conjugation available upon request

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