Thermo Fisher Scientific Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, TRITC
TRITC 형광으로 표지된 Goat anti-Rabbit IgG (H+L) 교차흡착 2차 항체. 면역세포화학(ICC/IF)에 최적화된 고순도 폴리클로날 항체. 다양한 종의 IgG에 대한 교차 반응 최소화. 연구용 형광 검출 및 시그널 증폭에 적합.
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Thermo Fisher Scientific Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, TRITC
Applications and Tested Dilution
- Immunocytochemistry (ICC/IF): 4 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Rabbit |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | TRITC |
| Excitation/Emission Max | 552/578 nm |
| Form | Lyophilized |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2534781 |
| Target | IgG |
| Cross Adsorption | Against bovine, human and mouse IgG |
| Antibody Form | Whole Antibody |
Product Specific Information
Rehydrate with 1.1 mL of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.)
Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Shelf life: 1 year from date of receipt.
Prepare working dilution prior to use and then discard.
Based on immunoelectrophoresis, no reactivity is observed to non-immunoglobulin rabbit serum proteins, serum proteins from bovine, human, or mouse, IgG from human or mouse.
Target Information
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting, or purification of its specified target.
Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence).
They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody.
Most commonly, secondary antibodies are generated by immunizing the host animal with pooled immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Available Conjugate Formats
- Alexa Fluor 350, 405, 430, 488, 514, 532, 546, 555, 568, 594, 633, 635, 647, 660, 680, 700, 750, 790
- AP, APC, Biotin, Biotin-XX, Cyanine3, Cyanine5
- DyLight 350, 405, 488, 550, 594, 633, 650, 680, 755, 800
- FITC, HRP, Pacific Blue, Pacific Orange, PE, PE-Alexa Fluor 610, PE-Alexa Fluor 647
- Rhodamine, Rhodamine Red-X, Texas Red, Texas Red-X
- Custom conjugation available upon request
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