Thermo Fisher Scientific Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Biotin
Goat anti-Rabbit IgG (H+L) Biotin-conjugated secondary antibody로 Western blot, ICC/IF, Flow cytometry, ELISA에 사용 가능. Rabbit IgG에 높은 특이성과 교차흡착 처리로 비특이적 결합 최소화. 높은 순도와 안정성을 제공하며 연구용으로 적합.
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- A16108
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:10,000–1:40,000 |
| Immunocytochemistry (ICC/IF) | 1:500–1:20,000 |
| Flow Cytometry (Flow) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Rabbit |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2534780 |
| Target | IgG |
| Cross Adsorption | Against bovine, human, and mouse IgG |
| Antibody Form | Whole Antibody |
Target Information
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful for detection, sorting, or purification of their specified target.
Secondary antibodies offer increased versatility, enabling use with various detection systems (HRP, AP, fluorescence) and provide greater sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
Most commonly, these antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Available Formats
This antibody is also available conjugated with various fluorophores and labels, including:
- Alexa Fluor series (350–790)
- DyLight series (350–800)
- FITC, HRP, APC, PE, TRITC, Texas Red, Rhodamine, and others
- Custom conjugation available upon request
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