Thermo Fisher Scientific RAD52 Monoclonal Antibody (5E11E7)
RAD52 단백질을 인식하는 Mouse Monoclonal Antibody로, WB, ICC/IF, Flow Cytometry, ELISA에 적합합니다. 정제된 인간 RAD52 단백질(AA 269-418)을 면역원으로 사용하였으며, Protein G로 정제된 액상형 항체입니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | - |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 | 1 publication |
| Flow Cytometry (Flow) | 1:200–1:400 | - |
| ELISA | 1:10,000 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5E11E7 |
| Immunogen | Purified recombinant fragment of human RAD52 (AA: 269–418) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C (short term). For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2787511 |
Product Specific Information
MA5-31888 has been tested in indirect ELISA.
Target Information
DNA double-strand breaks can be generated by exposure to ionizing radiation or through endogenous cellular reactions. These breaks are typically repaired through homologous recombination (HR) or non-homologous end joining (NHEJ).
Homologous recombination involves the RAD52 epistasis group, including RAD50, RAD51, RAD52, RAD54, RDH54/TID1, RAD55, RAD57, RAD59, MRE11, and XRS2.
RAD52 catalyzes strand annealing reactions and forms oligomers that attach to single-stranded DNA ends.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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