Thermo Fisher Scientific RAD52 Monoclonal Antibody (5E11B2)
RAD52 단백질을 인식하는 Mouse monoclonal antibody로 Western blot, Flow cytometry, ELISA에 사용 가능. 정제된 recombinant human RAD52 fragment(AA 269-418)를 항원으로 제작. Protein G 정제, PBS buffer, 1 mg/mL 농도. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Flow Cytometry (Flow) | 1:200–1:400 |
| ELISA | 1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5E11B2 |
| Immunogen | Purified recombinant fragment of human RAD52 (AA: 269–418) expressed in E. coli. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2787510 |
Product Specific Information
MA5-31887 has been tested in indirect ELISA.
Target Information
DNA double-strand breaks can be generated by exposure to ionizing radiation or through endogenous cellular reactions. These breaks are generally repaired via homologous recombination (HR) or non-homologous end joining (NHEJ).
Homologous recombination requires the activity of RAD52 epistasis group members, including RAD50, RAD51, RAD52, RAD54, RDH54/TID1, RAD55, RAD57, RAD59, MRE11, and XRS2.
RAD52 catalyzes strand annealing reactions and forms oligomers that attach to ends of single-stranded DNA.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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