Thermo Fisher Scientific IFN gamma Monoclonal Antibody (4S.B3), PE, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Available Formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 660
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395 / 496 / 615 / 661 / 737 / 805
• Brilliant Violet 650
• eFluor 450 / 506 / 660
• FITC
• PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• Request custom conjugation

Product Specific Information

The 4S.B3 monoclonal antibody reacts with interferon-gamma (IFNγ), a 17 kDa cytokine produced by activated T and NK cells with antiviral and antiparasitic activity. It acts synergistically with TNFα to inhibit cell proliferation and modulate immune responses across various cell types expressing IFNγ receptors.

• Applications Reported: Intracellular flow cytometric analysis
• Applications Tested: Validated by flow cytometry of activated human cells
• Recommended Usage: ≤ 0.25 µg/test in 100 µL final volume (10⁵–10⁸ cells/test)
• Excitation: 488–561 nm
• Emission: 578 nm
• Laser Compatibility: Blue, Green, Yellow-Green
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

IFNγ (Interferon gamma, Type II interferon) is a macrophage activation factor primarily produced by T-lymphocytes and NK cells in response to antigens and cytokines. It is a dimeric glycoprotein (~45 kDa) consisting of two 146-amino-acid subunits, showing species-specific biological activity.

Functions:

• Antiviral and antiproliferative activity
• Induction of MHC Class I and II
• Macrophage activation
• Enhanced immunoglobulin secretion by B cells
• Cytokine regulation via JAK-STAT signaling

Regulation:

• Upregulated by IL-2, FGF basic, EGF
• Downregulated by Vitamin D₃ or DMN
• Gene mutations linked to aplastic anemia

Human IFNγ does not cross-react with mouse IFNγ and shares ~40% sequence homology.

For Research Use Only. Not for diagnostic or therapeutic use. Not for resale without authorization.

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