
Thermo Fisher Scientific IFN gamma Monoclonal Antibody (4S.B3), PE, eBioscience
인간 IFN-γ를 인식하는 4S.B3 단클론 항체로, PE 형광 표지되어 유세포분석에 적합합니다. 활성화된 T세포 및 NK세포에서의 IFN-γ 검출에 사용되며, 0.25 µg/test 농도로 최적화되었습니다. 4°C 보관, 동결 금지.
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2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific IFN gamma Monoclonal Antibody (4S.B3), PE, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Flow Cytometry (Flow) | 0.25 µg/test | View 88 publications |
| Miscellaneous PubMed (Misc) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse, Rat, Rhesus monkey |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 4S.B3 |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1272169 |
Additional Available Formats
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 660
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395 / 496 / 615 / 661 / 737 / 805
- Brilliant Violet 650
- eFluor 450 / 506 / 660
- FITC
- PE-Cyanine7
- PE-eFluor 610
- PerCP-Cyanine5.5
- Request custom conjugation
Product Specific Information
The 4S.B3 monoclonal antibody reacts with interferon-gamma (IFNγ), a 17 kDa cytokine produced by activated T and NK cells with antiviral and antiparasitic activity. It acts synergistically with TNFα to inhibit cell proliferation and modulate immune responses across various cell types expressing IFNγ receptors.
- Applications Reported: Intracellular flow cytometric analysis
- Applications Tested: Validated by flow cytometry of activated human cells
- Recommended Usage: ≤ 0.25 µg/test in 100 µL final volume (10⁵–10⁸ cells/test)
- Excitation: 488–561 nm
- Emission: 578 nm
- Laser Compatibility: Blue, Green, Yellow-Green
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
IFNγ (Interferon gamma, Type II interferon) is a macrophage activation factor primarily produced by T-lymphocytes and NK cells in response to antigens and cytokines. It is a dimeric glycoprotein (~45 kDa) consisting of two 146-amino-acid subunits, showing species-specific biological activity.
Functions:
- Antiviral and antiproliferative activity
- Induction of MHC Class I and II
- Macrophage activation
- Enhanced immunoglobulin secretion by B cells
- Cytokine regulation via JAK-STAT signaling
Regulation:
- Upregulated by IL-2, FGF basic, EGF
- Downregulated by Vitamin D₃ or DMN
- Gene mutations linked to aplastic anemia
Human IFNγ does not cross-react with mouse IFNγ and shares ~40% sequence homology.
For Research Use Only. Not for diagnostic or therapeutic use. Not for resale without authorization.
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