Thermo Fisher Scientific PPARA Monoclonal Antibody (OTI1E11), TrueMAB
인간 PPARA 단백질(aa 145-420)을 인식하는 Mouse IgG1 단클론 항체. Western blot 및 Immunocytochemistry에 적합. 무보존제, 동결건조 형태로 제공되며, 재구성 후 1 mg/mL 농도 유지. 연구용 전용 제품.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500
Immunocytochemistry (ICC/IF)
- Tested Dilution: 1:100
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1E11 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 145–420 of human PPARA produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to reach a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
Peroxisome proliferators are non-genotoxic carcinogens acting through nuclear hormone receptors known as peroxisome proliferator-activated receptors (PPARs). These ligand-dependent intracellular proteins activate transcription by binding specific DNA sequences.
PPARs are activated by compounds such as clofibric acid, nafenopin, WY-14,643, and certain fatty acids. They can induce transcription of acyl-CoA oxidase and cytochrome P450 (CYP450) A6 via specific response elements.
PPARs form heterodimers with retinoid X receptor (RXR) alpha, similar to other nuclear hormone receptors.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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