Thermo Fisher Scientific BCL2L15 Polyclonal Antibody
Thermo Fisher Scientific의 BCL2L15 Polyclonal Antibody는 인간, 마우스, 랫트에 반응하는 Rabbit IgG 항체로, Western blot, IHC, ICC, Flow cytometry에 사용 가능합니다. 1 mg/mL 농도의 액상 형태이며 PBS 버퍼에 보관됩니다. 연구용으로만 사용됩니다.
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Applications
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent | |
| Immunocytochemistry (ICC/IF) | Assay-dependent | |
| Flow Cytometry (Flow) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein within Human BCL2L15 aa 1–163 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 0.2% BSA, 50% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2890421 |
Target Information
Proteins of the Bcl-2 family are critical regulators of apoptosis. Proapoptotic members, like Bax, contain three of the four Bcl-2 homology regions (BH1–3), while BH3-only proteins, like Bim, possess only the short BH3 motif. Database searches revealed Bfk, an unusual novel member of the Bcl-2 family that contains BH2 and BH3 regions but not BH1 or BH4. Bfk is most closely related to Bcl-G(L). It lacks a C-terminal membrane anchor and is cytosolic. Enforced expression of Bfk weakly promoted apoptosis and antagonized Bcl-2’s prosurvival function. Like Bcl-G(L), Bfk did not bind to any Bcl-2 family members, even though its BH3 motif can mediate association with prosurvival proteins. Low amounts of Bfk were found in stomach, ovary, bone marrow, and spleen, but its level in the mammary gland rose markedly during pregnancy, suggesting a role in mammary development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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