Thermo Fisher Scientific Human MMP-9 Recombinant Protein

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Control (Ctrl)

Assay-dependent

-

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species

Human

Published species

Not Applicable

Expression System

E. coli

Amino acid sequence

Amino acids Asp113- Glu450 containing an N-terminal His-tag

Tag

His-tag

Molecular weight

42.03 kDa

Class

Recombinant

Type

Protein

Purity

>95%, by SDS-PAGE under reducing conditions

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.27 mg/mL

Purification

purified

Storage buffer

20mM tris HCl, pH 8.0, with 50% glycerol

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

Product Specific Information

This product contains purified recombinant human MMP-9 protein with an N-terminal hexahistidine tag. The recombinant protein is a fragment corresponding to the catalytic domain of the protein (residues 113-450).

This product may be used as a positive control in many immunological assays.

Target Information

MMP9 (matrix metallopeptidase 9, GELB, CLG4B) is a matrix metalloproteinase, a family of zinc and calcium-dependent endopeptidases that degrade extracellular matrix proteins. MMP9 is secreted as a 92kDa zymogen and cleavage of pro-MMP9 results in the active enzyme with a molecular weight of 82kDa. MMP9 has a gelatin-binding domain consisting of three fibronectin type II units, a catalytic domain containing the zinc-binding site, a proline-rich type V collagen-homologous domain and a hemopexin-like domain. MMP9 is produced by monocytes, macrophages, neutrophils, keratinocytes, fibroblasts, osteoclasts and endothelial cells, and is involved in inflammatory responses, tissue remodeling, wound healing, tumor growth and metastasis. MMP9 is supplied by bone marrow-derived cells and contributes to skin carcinogenesis. Further, MMP9 degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. Studies have shown that elevated MMP9 is associated with progression of idiopathic atrial fibrillation and aortic aneurysm.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.