Thermo Fisher Scientific HLA-A3 Monoclonal Antibody (GAP.A3), APC, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.25 µg)/test
• Publications: View 4 publications

Product Specifications

Additional Formats Available

• FITC
• NovaFluor Blue 510
• NovaFluor Blue 585
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Red 710
• NovaFluor Red 725
• NovaFluor Yellow 570
• NovaFluor Yellow 660
• NovaFluor Yellow 730
• NovaFluor Yellow 755
• PE
• Super Bright 436
• Super Bright 600
• Request custom conjugation

Product Specific Information

The monoclonal antibody GAP.A3 reacts with the human major histocompatibility complex (MHC) class I molecule HLA-A3, a member of the HLA-A family.
HLA-A3 exists as two subtypes (HLA-A3.1 and HLA-A3.2) differing at amino acid positions 152 and 156.
MHC class I molecules are expressed on all nucleated cells and associate with β2-microglobulin. They present intracellular peptides to cytotoxic T lymphocytes.
HLA-A3 is expressed by 15–25% of cancer patients and can bind the tumor-associated antigen human telomerase reverse transcriptase (hTERT).

• Applications Reported: Flow cytometry
• Applications Tested: Pre-titrated and tested using normal human peripheral cells at 5 µL (0.25 µg)/test
• Excitation: 633–647 nm
• Emission: 660 nm
• Laser: Red Laser
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

HLA-A belongs to the HLA class I heavy chain paralogues, forming a heterodimer with β2-microglobulin.
The heavy chain (~45 kDa) is membrane-anchored and encoded by 8 exons:

• Exon 1: leader peptide
• Exons 2–3: α1, α2 domains (peptide-binding)
• Exon 4: α3 domain
• Exon 5: transmembrane region
• Exons 6–7: cytoplasmic tail

Polymorphisms in exons 2 and 3 determine peptide binding specificity.
HLA typing is routinely performed for bone marrow and kidney transplantation.
HLA and MHC antibodies are important in Immunopeptidomics, aiding in neoantigen identification via LC-MS/MS.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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