Thermo Fisher Scientific VSIG4 Monoclonal Antibody (NLA14), APC, eBioscience
마우스 VSIG4 단백질을 인식하는 NLA14 단클론 항체로, APC 형광체가 결합된 제품입니다. 유세포분석(Flow Cytometry)에 최적화되어 있으며, 0.5 µg/test 권장 농도로 사용합니다. 4°C 암소 보관, 동결 금지. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.5 µg/test | View 6 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), APC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | NLA14 |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2637428 |
Available Formats
- Alexa Fluor 488
- PE
- PE-Cyanine7
- PE-eFluor 610
- Super Bright 436
- Super Bright 600
- Request custom conjugation
Product Specific Information
This NLA14 monoclonal antibody recognizes mouse V-Set and Immunoglobulin domain containing 4 (VSIG4), also known as Complement Receptor of the Immunoglobulin superfamily (CRIg) or Z39Ig.
VSIG4 is a type I transmembrane glycoprotein structurally related to the B7 family of immune regulatory proteins. It contains one complete V-type Ig domain and one truncated C-type Ig domain.
VSIG4 is exclusively expressed on tissue-resident and tumor-infiltrating macrophages. It binds complement components C3b and iC3b, inhibiting the alternative complement pathway and promoting phagocytosis of complement-opsonized pathogens.
It also suppresses T cell activation, proliferation, and IL-2 production, contributing to peripheral T cell tolerance and inflammation suppression. Expression on tumor-infiltrating macrophages suggests a role in immune evasion. Pro-inflammatory stimuli such as TNF and LPS down-regulate VSIG4 expression.
Peritoneal macrophages in Balb/c mice express higher VSIG4 levels than those in C57Bl/6 or Swiss Webster mice.
The NLA14 antibody recognizes VSIG4 on formaldehyde-fixed and permeabilized cells. It does not block VSIG4 ligation to its T cell ligand and does not cross-react with rat or human VSIG4.
Applications Reported: Flow cytometric analysis
Applications Tested: Tested by flow cytometric analysis of mouse peritoneal exudate cells. Recommended ≤0.5 µg/test (100 µL final volume, 10⁵–10⁸ cells/test). Optimize concentration for best performance.
Excitation: 633–647 nm
Emission: 660 nm
Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered
Target Information
T cell activation by antigen-presenting cells (APCs) is regulated by the B7 family. VSIG4 acts as a strong negative regulator of murine and human T cell proliferation and IL-2 production.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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