Thermo Fisher Scientific CD29 (Integrin beta 1) Monoclonal Antibody (eBioHMb1-1 (HMb1-1)), APC, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats Available

• Unconjugated
• APC-eFluor 780
• Biotin
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 660-120S
• NovaFluor Red 685
• NovaFluor Red 700
• NovaFluor Red 755
• NovaFluor Yellow 660
• NovaFluor Yellow 700
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Request custom conjugation

Product Specific Information

Description:
The eBioHMb1-1 monoclonal antibody reacts with mouse and rat CD29 (integrin beta 1), a 110–120 kDa member of the beta integrin family expressed by leukocytes, endothelial, smooth muscle, and epithelial cells. CD29 binds non-covalently with alpha integrins (CD49a–f) to form VLA-1 through VLA-6 complexes, as well as with CD51. These heterodimers mediate cellular adhesion, trafficking, proliferation, and differentiation. CD29-containing integrins bind to extracellular matrix proteins such as collagen, laminin, fibronectin, and vitronectin, and also interact with cellular receptors like VCAM-1 and MadCAM-1.

Applications Reported:
This antibody is reported for use in flow cytometric analysis.

Applications Tested:
Tested by flow cytometric analysis of mouse bone marrow cells. Recommended ≤0.25 µg/test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test. Optimize titration for best performance.

Excitation/Emission: 633–647 nm / 660 nm
Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Gene: ITGB1 (Integrin Subunit Beta 1, CD29)
Protein Size: 110 kDa cell surface glycoprotein

ITGB1 forms heterodimers with multiple alpha chains (α1–α6), defining VLA integrins that mediate cell adhesion to extracellular matrix proteins. It is expressed in various cell types including leukocytes and platelets. Decreased ITGB1 expression correlates with multidrug resistance in tumor cells. ITGB1 is involved in immune cell trafficking, tissue repair, and inflammatory responses. Two major isoforms, β1A and β1D, show tissue-specific distribution, with β1A in most tissues and β1D in cardiac and skeletal muscle.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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