Thermo Fisher Scientific KSR1 Polyclonal Antibody
KSR1 단백질을 인식하는 Rabbit Polyclonal Antibody로, Western blot 및 Immunocytochemistry에 적합. 인간, 마우스, 랫트에 반응하며, 고순도의 affinity chromatography로 정제됨. PBS 기반 버퍼에 보관되며 연구용으로만 사용 가능.
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Applications
Western Blot (WB)
- Tested Dilution: 1:1,000–1:3,000
Immunocytochemistry (ICC/IF)
- Tested Dilution: 1:100–1:500
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human KSR1 (Accession Q8IVT5), corresponding to amino acid residues R544–R594 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2852293 |
Product Specific Information
This antibody detects endogenous levels of total KSR1.
Target Information
KSR1 is part of a multiprotein signaling complex that promotes phosphorylation of Raf family members and activation of downstream MAP kinases. Independently of its kinase activity, it acts as a MAP2K1/MEK1 and MAP2K2/MEK2-dependent allosteric activator of BRAF. Upon binding to MAP2K1/MEK1 or MAP2K2/MEK2, KSR1 dimerizes with BRAF and promotes BRAF-mediated phosphorylation of MAP2K1/MEK1 and/or MAP2K2/MEK2 (PubMed:29433126). It promotes activation of MAPK1 and/or MAPK3 in response to EGF and cAMP. Although some kinase activity has been detected in vitro, its physiological relevance remains uncertain. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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