Thermo Fisher Scientific CD335 (NKp46) Monoclonal Antibody (29A1.4), PerCP-eFluor 710, eBioscience
마우스 NKp46(CD335)을 인식하는 단클론 항체로 NK 세포 분석에 적합. PerCP-eFluor 710 형광체로 결합되어 488 nm에서 여기, 710 nm에서 방출. Flow cytometry용으로 최적화되어 있으며 다양한 마우스 균주에 적용 가능. 4°C 암소 보관, 동결 금지.
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Thermo Fisher Scientific CD335 (NKp46) Monoclonal Antibody (29A1.4), PerCP-eFluor 710, eBioscience
Applications and Tested Dilution
- Application: Flow Cytometry (Flow)
- Tested Dilution: 0.25 µg/test
- Publications: View 22 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), PerCP-eFluor™ 710, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 29A1.4 |
| Conjugate | PerCP-eFluor™ 710 |
| Excitation/Emission Max | 482 / 708 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1834442 |
Available Formats
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant Violet 711
- eFluor 450
- eFluor 660
- FITC
- Functional Grade
- PE
- PE-Cyanine7
- PE-eFluor 610
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Request custom conjugation
Product Specific Information
The monoclonal antibody 29A1.4 recognizes mouse NKp46 (CD335), a member of the natural cytotoxicity receptor (NCR) family. CD335 is a glycoprotein with two Ig-like domains and a short cytoplasmic tail. Expression is uniquely found on NK cells, including immature NK cells (DX5-, CD3-) allowing discrimination between NK and NKT cells (NKp46+, CD3-).
Staining is not strain-specific and has been confirmed in C57Bl/6, SJL, CBA/CA, and BALB/C mice. NKp46 plays a role in NK cell-mediated lysis of tumor and pathogen-infected cells.
- Activation: 29A1.4 activates NK cells in vitro but does not deplete NK cells in vivo.
- Applications Reported: Flow cytometric analysis.
- Applications Tested: Mouse spleen cells; ≤ 0.25 µg/test (100 µL final volume).
- Cell Range: 10⁵–10⁸ cells/test; titration recommended for optimal performance.
Fluorochrome Information:
PerCP-eFluor® 710 can replace PE-Cy5, PE-Cy5.5, or PerCP-Cy5.5.
- Emission: 710 nm
- Excitation: 488 nm (Blue Laser)
- Recommended Filters: 685 LP dichroic mirror & 710/40 band pass filter (695/40 acceptable).
Conjugated antibodies are stable after exposure to freshly prepared 2% formaldehyde overnight at 4°C.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
NKp46 (CD335, NCR1) is a cytotoxicity-activating receptor contributing to NK cell-mediated tumor lysis efficiency. It consists of two Ig-like domains similar to leukocyte immunoglobulin-like (LIR) and killer inhibitory receptors (KIR).
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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