Thermo Fisher Scientific CD335 (NKp46) Monoclonal Antibody (29A1.4), PerCP-eFluor 710, eBioscience

Thermo Fisher Scientific CD335 (NKp46) Monoclonal Antibody (29A1.4), PerCP-eFluor 710, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.25 µg/test
• Publications: View 22 publications

Product Specifications

Available Formats

• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant Violet 711
• eFluor 450
• eFluor 660
• FITC
• Functional Grade
• PE
• PE-Cyanine7
• PE-eFluor 610
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Request custom conjugation

Product Specific Information

The monoclonal antibody 29A1.4 recognizes mouse NKp46 (CD335), a member of the natural cytotoxicity receptor (NCR) family. CD335 is a glycoprotein with two Ig-like domains and a short cytoplasmic tail. Expression is uniquely found on NK cells, including immature NK cells (DX5-, CD3-) allowing discrimination between NK and NKT cells (NKp46+, CD3-).
Staining is not strain-specific and has been confirmed in C57Bl/6, SJL, CBA/CA, and BALB/C mice. NKp46 plays a role in NK cell-mediated lysis of tumor and pathogen-infected cells.

• Activation: 29A1.4 activates NK cells in vitro but does not deplete NK cells in vivo.
• Applications Reported: Flow cytometric analysis.
• Applications Tested: Mouse spleen cells; ≤ 0.25 µg/test (100 µL final volume).
• Cell Range: 10⁵–10⁸ cells/test; titration recommended for optimal performance.

Fluorochrome Information:
PerCP-eFluor® 710 can replace PE-Cy5, PE-Cy5.5, or PerCP-Cy5.5.

• Emission: 710 nm
• Excitation: 488 nm (Blue Laser)
• Recommended Filters: 685 LP dichroic mirror & 710/40 band pass filter (695/40 acceptable).
  Conjugated antibodies are stable after exposure to freshly prepared 2% formaldehyde overnight at 4°C.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

NKp46 (CD335, NCR1) is a cytotoxicity-activating receptor contributing to NK cell-mediated tumor lysis efficiency. It consists of two Ig-like domains similar to leukocyte immunoglobulin-like (LIR) and killer inhibitory receptors (KIR).

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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