Thermo Fisher Scientific CD336 (NKp44) Monoclonal Antibody (44.189), PerCP-eFluor 710, eBioscience
인간 CD336(NKp44/NCR2)을 인식하는 단클론 항체로, PerCP-eFluor 710 형광체로 표지됨. NK 세포 활성화 및 종양 연구용 Flow Cytometry에 적합. 488 nm 레이저에서 여기, 710 nm 방출. 4°C 암소 보관, 동결 금지.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Flow Cytometry (Flow) | 5 µL (0.25 µg)/test | View 1 publication |
| In vitro Assay (IV) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Recommended Isotype Control | Mouse IgG2b kappa Isotype Control (eBMG2b), PerCP-eFluor™ 710, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 44.189 |
| Conjugate | PerCP-eFluor™ 710 |
| Excitation / Emission Max | 482 / 708 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2573749 |
Additional Formats:
Product Specific Information
The monoclonal antibody 44.189 reacts with human CD336 (NKp44/NCR2), a 44-kDa transmembrane glycoprotein of the natural cytotoxicity receptor (NCR) family containing an ITIM domain. CD336 expression and NK cell activation require association with DAP12, an ITAM-containing accessory protein.
Originally thought to be expressed only on IL-2-activated NK cells and some γδ T lymphocytes, CD336 has also been identified on IL-3-stimulated plasmacytoid dendritic cells, αβ T cells in the small intestinal epithelium, and T cells in cord blood. It plays a role in the innate immune response against viral infections and tumor proliferation.
The 44.189 antibody facilitates CD336 crosslinking and is validated for flow cytometric analysis. It is pre-titrated and tested using IL-2-stimulated normal human peripheral blood cells at 5 µL (0.25 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).
Fluorochrome Information:
- PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm).
- Recommended filter: 710/50 bandpass (695/40 acceptable alternative).
- Ensure instrument compatibility before use.
Fixation:
Samples can be stored up to 3 days at 4°C in the dark using:
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD336 (NKp44/NCR2) is a natural cytotoxicity receptor involved in NK cell-mediated immune responses. It associates with DAP12 for activation signaling and is expressed on IL-2-activated NK cells, subsets of T lymphocytes, and dendritic cells. It contributes to antiviral defense and tumor suppression mechanisms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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