Thermo Fisher Scientific Nitrotyrosine Polyclonal Antibody
Nitrotyrosine을 표적으로 하는 Rabbit Polyclonal Antibody로, Western blot, IHC, ICC, Flow cytometry 및 ELISA에 사용 가능. 단백질 산화 손상 및 염증 연구용으로 적합하며, 다양한 질환 모델에서 NO 관련 세포 손상 마커 분석에 활용됨.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:300–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:200 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:100 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated to Nitrotyrosine |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Nitrotyrosine is a relatively stable product formed from various reaction pathways, notably from the reaction of peroxynitrite (formed from superoxide and nitric oxide radicals) with tyrosine. As a strong oxidant and nitrating agent, peroxynitrite mediates tyrosine nitration reactions on proteins, resulting in the inactivation of certain enzymes such as alpha1-antiproteinase and antioxidant enzymes like catalase and superoxide dismutase (SOD).
Nitrotyrosine serves as an indicator of cell damage and inflammation, as well as nitric oxide (NO) production. It is considered a marker for NO-induced cellular damage and has been implicated in the pathogenesis of several inflammatory, infectious, and degenerative diseases, including Alzheimer’s and Parkinson’s disease, multiple sclerosis, amyotrophic lateral sclerosis (ALS), asthma, and atherosclerosis. Tyrosine nitration at the protein level can lead to loss or alteration of protein function, as demonstrated for proteins such as p53 and manganese-dependent superoxide dismutase (MnSOD).
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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