Thermo Fisher Scientific alpha GalCer:CD1d Complex Monoclonal Antibody (L363), Functional Grade, eBioscience
alpha-galactosylceramide:CD1d 복합체를 인식하는 L363 단클론 항체로 iNKT 세포 활성화 연구에 적합. Flow cytometry 및 기능적 분석에 사용 가능하며, 1 mg/mL 농도의 액상 형태로 제공. 고순도(>90%) 및 낮은 엔도톡신 수준(<0.001 ng/µg).
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.25 µg/test | View 4 publications |
| Functional Assay (Functional) | Assay-Dependent | View publications |
Product Specifications
| Specification | Detail |
|---|---|
| Published Species | Mouse |
| Host / Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), Functional Grade, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | L363 |
| Conjugate | Functional Grade |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2865920 |
Additional Formats
Product Specific Information
This L363 monoclonal antibody reacts with alpha-galactosylceramide bound to the mouse MHC class I-like protein CD1d. This complex is recognized by invariant natural killer T (iNKT) cells, a subset of T lymphocytes that expresses the invariant TCR alpha chain Valpha14Jalpha18. Activation of iNKT cells leads to production of Th1 and Th2-associated cytokines as well as dendritic cell maturation.
The L363 antibody has been reported to neutralize iNKT cell activation by alpha-galactosylceramide:CD1d complexes.
Applications Reported: Functional assays
Applications Tested: Flow cytometric analysis of alpha-galactosylceramide-loaded mouse CD1d-transfected cells.
Recommended usage ≤ 0.25 µg per test (100 µL final volume). Cell number may range from 10⁵ to 10⁸ cells/test. Titrate carefully for optimal performance.
Storage and Handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: >90% (SDS-PAGE).
Endotoxin Level: <0.001 ng/µg antibody (LAL assay).
Aggregation: <10% (HPLC).
Target Information
This alpha-galactosylceramide:CD1d complex is recognized by invariant natural killer T (iNKT) cells expressing the invariant TCR alpha chain Va14Ja18. Activation of iNKT cells leads to production of Th1 and Th2 cytokines and dendritic cell maturation.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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배송/결제/교환/반품 안내
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