Thermo Fisher Scientific CD197 (CCR7) Monoclonal Antibody (4B12), Functional Grade, eBioscience
CD197(CCR7) 단클론 항체(4B12)는 마우스 CCR7을 인식하는 Functional Grade 항체로, Flow Cytometry 및 Immunoprecipitation에 적합합니다. 림프구 이동 연구, T세포 분화 및 면역 반응 연구에 활용되며, 37°C 염색 시 높은 신호 강도를 제공합니다.
- 카탈로그번호
- 16-1971-xxx (4개 옵션)
- 판매단위
- pk
카탈로그
4개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD197 (CCR7) Monoclonal Antibody (4B12), Functional Grade, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | 1 |
| Immunocytochemistry (ICC/IF) | - | 1 |
| Flow Cytometry (Flow) | 1 µg/test | 37 |
| Immunoprecipitation (IP) | Assay-Dependent | - |
| Neutralization (Neu) | - | 1 |
| Functional Assay (Functional) | Assay-Dependent | 1 |
| Control (Ctrl) | Assay-Dependent | - |
| Inhibition Assays (IA) | - | 1 |
| Miscellaneous PubMed (Misc) | - | 1 |
Product Specifications
| Specification | Detail |
|---|---|
| Published species | Human, Mouse |
| Host/Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Functional Grade |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 4B12 |
| Conjugate | Functional Grade |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage conditions | 4°C |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2865911 |
Available Conjugates:
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- eFluor 450
- PE
- PE-Cyanine7
- PE-eFluor 610
- PerCP-Cyanine5.5
- Custom conjugation available upon request
Product Specific Information
Description:
The 4B12 monoclonal antibody reacts with mouse CCR7 (CD197), a chemokine receptor for CCL19 and CCL21. CCR7 mediates lymphocyte migration to secondary lymphoid organs and plays roles in dendritic cell and B cell localization during immune responses. It is also a marker for central memory T cells (TCM) and is involved in thymocyte development.
Applications Reported:
- Flow Cytometric Analysis
- Immunoprecipitation
Applications Tested:
Tested by flow cytometric analysis of mouse splenocytes and thymocytes. Recommended ≤1 µg/test in 100 µL volume. Optimize titration for best performance.
Staining Protocol (4B12 Antibody)
- Prepare cell suspension and block FcγR with 5 µg/million cells anti-mouse CD16/32 for 15 min on ice.
- Without washing, add 1 µg/million cells 4B12 and incubate at 37°C (preferred) or 2–8°C for 30 min.
- Wash cells once with 3 mL Flow Cytometry Staining Buffer and decant.
- Add secondary antibody and incubate at 2–8°C for 30 min.
- Wash once more and analyze by flow cytometry.
Note:
- Staining at 37°C yields brighter signal and better resolution.
- Co-staining with CCL19-Fc may be hindered due to steric interference.
- 4B12 binding can block CCL19-Fc binding, but not vice versa.
Additional Information
| Property | Specification |
|---|---|
| Filtration | 0.2 µm post-manufacturing filtered |
| Purity | >90% (SDS-PAGE) |
| Endotoxin Level | <0.001 ng/µg antibody (LAL assay) |
| Aggregation | <10% (HPLC) |
| Storage and Handling | Use in sterile environment |
Target Information
CCR7 is a G protein-coupled chemokine receptor induced by Epstein-Barr virus (EBV) and expressed in blood, bone marrow, lymph node, and intestine. It regulates migration of memory T cells, dendritic cell maturation, and lymphocyte trafficking to inflamed tissues. CCR7 ligands include CCL19 (ELC) and CCL21 (SLC), essential for immune cell chemotaxis.
For Research Use Only. Not for diagnostic use or resale without authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.