Thermo Fisher Scientific CD197 (CCR7) Monoclonal Antibody (4B12), Functional Grade, eBioscience

Applications and Tested Dilution

Product Specifications

Available Conjugates:

• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• eFluor 450
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• Custom conjugation available upon request

Product Specific Information

Description:
The 4B12 monoclonal antibody reacts with mouse CCR7 (CD197), a chemokine receptor for CCL19 and CCL21. CCR7 mediates lymphocyte migration to secondary lymphoid organs and plays roles in dendritic cell and B cell localization during immune responses. It is also a marker for central memory T cells (TCM) and is involved in thymocyte development.

Applications Reported:

• Flow Cytometric Analysis
• Immunoprecipitation

Applications Tested:
Tested by flow cytometric analysis of mouse splenocytes and thymocytes. Recommended ≤1 µg/test in 100 µL volume. Optimize titration for best performance.

Staining Protocol (4B12 Antibody)

1. Prepare cell suspension and block FcγR with 5 µg/million cells anti-mouse CD16/32 for 15 min on ice.
2. Without washing, add 1 µg/million cells 4B12 and incubate at 37°C (preferred) or 2–8°C for 30 min.
3. Wash cells once with 3 mL Flow Cytometry Staining Buffer and decant.
4. Add secondary antibody and incubate at 2–8°C for 30 min.
5. Wash once more and analyze by flow cytometry.

Note:

• Staining at 37°C yields brighter signal and better resolution.
• Co-staining with CCL19-Fc may be hindered due to steric interference.
• 4B12 binding can block CCL19-Fc binding, but not vice versa.

Additional Information

Target Information

CCR7 is a G protein-coupled chemokine receptor induced by Epstein-Barr virus (EBV) and expressed in blood, bone marrow, lymph node, and intestine. It regulates migration of memory T cells, dendritic cell maturation, and lymphocyte trafficking to inflamed tissues. CCR7 ligands include CCL19 (ELC) and CCL21 (SLC), essential for immune cell chemotaxis.

For Research Use Only. Not for diagnostic use or resale without authorization.