Thermo Fisher Scientific FBXL20 Polyclonal Antibody
Human FBXL20 단백질을 인식하는 Rabbit Polyclonal Antibody. Western blot 및 IHC(P) 검증 완료. 항원 친화 크로마토그래피로 정제된 액상 항체로, PBS(40% glycerol) 버퍼에 보관. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human FBXL20. Recombinant protein control fragment (Product #RP-100020) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2641532 |
Product Specific Information
Immunogen sequence:
MRRDVNGVTK SRFEMFSNSD EAVINK
Highest antigen sequence identity:
- Mouse: 100%
- Rat: 100%
Target Information
Members of the F-box protein family, such as Scrapper, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1, cullin, and F-box proteins, act as protein-ubiquitin ligases. Scrapper is selectively expressed in the brain, broadly expressed within the mouse CNS, and is abundant at the presynaptic membrane. Scrapper has orthologs in C. elegans, D. melanogaster, and mammals, suggesting it functions as an important membrane-localized E3 ligase across species. Scrapper acts through RIM1a degradation via the ubiquitin-proteasome system (UPS) pathway to regulate synaptic transmission, highlighting protein degradation as a mechanism for maintaining synaptic communication balance.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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