Thermo Fisher Scientific ORC1 Monoclonal Antibody (7A7)
Human ORC1 단백질을 인식하는 Rat monoclonal 항체로, Western blot 및 Immunoprecipitation에 사용 가능. 비결합(unconjugated) 액상 형태이며 1 mg/mL 농도. 단기 4°C, 장기 -20°C 보관 권장. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Rat / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 7A7 |
| Immunogen | Full length human ORC1 protein (1–861 aa, UniProt ID: Q13415-1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2538497 |
Product Specific Information
- MA5-17025 reacts with ORC1 in human samples.
- Successfully used in immunoprecipitation and Western blot applications.
- The immunogen is ORC1 (hsORC) produced in P3-x63Ag.8.653 cells.
Target Information
The origin recognition complex (ORC) is a highly conserved six-subunit protein complex essential for the initiation of DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins.
The protein encoded by this gene is the largest subunit of the ORC complex. While other ORC subunits are stable throughout the cell cycle, the levels of this protein vary during the cycle, controlled by ubiquitin-mediated proteolysis after initiation of DNA replication. This protein is selectively phosphorylated during mitosis and interacts with MYST histone acetyltransferase 2 (MyST2/HBO1), a protein involved in transcriptional silencing control.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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