Thermo Fisher Scientific PD-1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1-4 µg/mL

View 1 publication 1 publication

Immunohistochemistry (IHC)

-

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

2.5-5 µg/mL

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A 16 amino acid peptide from near the carboxy terminus of human PD-1. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PD-1 (CD279),uniProtId:Q15116-1,ncbiNodeId:9606,antigenRange:288,antigenLength:288,antigenImageFileName:PA5-20350_PD-1_CD279_Q15116-1_Rabbit.svg,antigenImageFileNamePDP:PA5-20350_PD-1_CD279_Q15116-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11153220

Product Specific Information

Despite its predicted molecular weight, PD-1 often migrates at a higher molecular weight in SDS-PAGE. A suggested positive control is THP-1 cell lysate.

PA5-20350 can be used with blocking peptide PEP-0470.

Target Information

Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of danger signals such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD1- mice who exhibit hyperactivated immune systems and autoimmune diseases. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.