Thermo Fisher Scientific CD1a Monoclonal Antibody (SP157)
Human CD1a 단백질을 인식하는 Rabbit monoclonal antibody (Clone SP157). IHC(Paraffin)용으로 1:100 희석에서 검증됨. 열매개 항원 회복 권장. Thymus 또는 피부 조직을 양성 대조로 사용 가능. 연구용으로만 사용.
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Applications
- Immunohistochemistry (Paraffin) (IHC (P)): Tested dilution 1:100
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SP157 |
| Immunogen | Synthetic peptide derived from the internal region of human CD1a protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.6, with 1% BSA |
| Contains | <0.1% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2537852 |
Product Specific Information
Heat-mediated antigen retrieval is recommended prior to staining using a 1 mM EDTA buffer (pH 8.0) for 10 minutes, followed by cooling at room temperature for 20 minutes.
After antigen retrieval, incubate samples with the primary antibody for 10 minutes at room temperature.
A suggested positive control is thymus or skin tissue.
Target Information
CD1a is a non-polymorphic MHC Class I-related cell surface glycoprotein expressed in association with beta-2 microglobulin.
It is found on cortical thymocytes, Langerhans cells, dendritic cells, certain T-cell leukemias, and other tissues.
CD1a mediates the presentation of lipid and glycolipid antigens of self or microbial origin to T cells.
The human genome contains five CD1 family genes (CD1a, b, c, d, e) organized on chromosome 1.
CD1a localizes to the plasma membrane and recycling vesicles of the early endocytic system.
Alternatively spliced transcript variants of CD1a have been observed, but their biological validity has not been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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