Thermo Fisher Scientific CD138 Monoclonal Antibody (SP152)

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100

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Immunocytochemistry (ICC/IF)

1:50

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Product Specifications

Species Reactivity

Human

Published species

Not Applicable

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

SP152

Immunogen

Recombinant human CD138 protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.018 mg/mL

Purification

Protein A/G

Storage buffer

PBS, pH 7.6, with 1% BSA

Contains

0.1% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2537850

Product Specific Information

Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is tonsil tissue.

Target Information

CD138 (syndecan 1) is a transmembrane proteoglycan that can bind a variety of cytokines and modulate their activity, as well as the activity of extracellular matrix components and influence many developmental processes. CD138 is expressed mainly in differentiating keratinocytes and is transiently upregulated in all layers of the epidermis upon tissue injury. It is also highly expressed on plasma cells and can be detected even on fibroblasts, vascular smooth muscle cells and endothelial cells. Up-regulation and down-regulation of CD138 on the cell surface often correlates with the gain of cancerous characteristics. Serum levels of the shedded soluble sCD138 are used as a prognostic factor of cancerogenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.