Thermo Fisher Scientific CD23 Monoclonal Antibody (SP23)
Thermo Fisher Scientific의 CD23 Monoclonal Antibody (SP23)는 인간 CD23 단백질을 인식하는 rabbit monoclonal 항체로, Western blot, IHC, Flow Cytometry에 적합합니다. Heat-mediated antigen retrieval 권장. 연구용으로만 사용됩니다.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | Assay-dependent | - |
| Immunohistochemistry (IHC) | - | View 3 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 | View 1 publication |
| Flow Cytometry (Flow) | 1:100 | - |
Product Specifications
| Specification | Description |
|---|---|
| Published species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SP23 |
| Immunogen | Recombinant fragment within Human CD23 aa 1-250 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not Determined |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Wet ice |
| RRID | AB_2537854 |
Product Specific Information
Heat-mediated antigen retrieval is recommended prior to staining, using a 10 mM citrate buffer (pH 6.0) for 10 minutes followed by cooling at room temperature for 20 minutes.
Following antigen retrieval, incubate samples with primary antibody for 30 minutes at room temperature.
A suggested positive control is tonsil tissue.
Target Information
CD23 is a 45 kDa glycoprotein present on a subpopulation of freshly isolated peripheral blood and tonsil B cells and strongly expressed on EBV-transformed B lymphoblasts.
It is identical to the low-affinity IgE receptor found on B cells. Expression of CD23 has been detected in neoplastic cells from cases of B cell chronic lymphocytic leukemia and some cases of centroblastic/centrocytic lymphoma.
Functionally, CD23 is involved in B cell growth, differentiation, and IgE production.
CD23 also has a soluble form that acts as a potent mitogenic factor.
Diseases associated with CD23 dysfunction include chronic conjunctivitis and chronic lymphocytic leukemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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