Thermo Fisher Scientific BCKDHA Recombinant Rabbit Monoclonal Antibody (HL2405)
BCKDHA 단백질을 인식하는 토끼 유래 재조합 단클론 항체. Western blot, IHC, ICC 등 다양한 응용에 적합. HEK293 세포에서 발현된 IgG 형식의 항체로, Protein A로 정제됨. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent | |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | HL2405 |
| Immunogen | Recombinant fragment of human BCKDHA |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C and avoid freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_3092933 |
Target Information
The BCKDHA gene encodes the E1-alpha subunit of the branched-chain alpha-keto acid (BCAA) dehydrogenase complex (BCKD; EC 1.2.4.4), an inner-mitochondrial enzyme complex that catalyzes the oxidative decarboxylation of the branched-chain alpha-ketoacids derived from isoleucine, leucine, and valine.
This reaction is the second major step in the catabolism of branched-chain amino acids (Wynn et al., 1998).
The BCKD complex consists of three catalytic components:
- A heterotetrameric (α2β2) branched-chain alpha-keto acid decarboxylase (E1)
- A homo-24-meric dihydrolipoyl transacylase (E2)
- A homodimeric dihydrolipoamide dehydrogenase (E3)
E1 is a thiamine pyrophosphate (TPP)-dependent enzyme. The reaction is irreversible and constitutes the first committed step in BCAA oxidation. The BCKDHB gene encodes the beta subunit of E1. The complex also contains two regulatory enzymes, a kinase and a phosphorylase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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