Thermo Fisher Scientific ID3 Monoclonal Antibody (2B11)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-5 µg/mL

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Immunocytochemistry (ICC/IF)

1:100-1:1,000

-

Immunoprecipitation (IP)

1-5 µg/mL

View 1 publication 1 publication

ChIP assay (ChIP)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Not Applicable

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

2B11

Immunogen

The full-length (amino acids 1-119) mouse Id3 gene expressed in E. coli. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:ID3,uniProtId:P41133-1,ncbiNodeId:10090,antigenRange:1-119,antigenLength:119,antigenImageFileName:MA1-23242_ID3_P41133-1_House_mouse.svg,antigenImageFileNamePDP:MA1-23242_ID3_P41133-1_House_mouse_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2.45 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2295760

Product Specific Information

Recommended positive controls: Id3-transfected 293T, Id3 transfected Sol8 muscle cells and recombinant fusion protein.

Target Information

Members of the Id family of basic helix-loop-helix (bHLH) proteins include ID1, ID2, ID3 and ID4. They are ubiquitously expressed and dimerize with members of the class A and B HLH proteins. Due to the absence of the basic region, the resulting heterodimers cannot bind DNA. The ID-type proteins thus appear to negatively regulate DNA binding of bHLH proteins. Since ID1 inhibits DNA binding of E12 and Myo D, it apparently functions to inhibit muscle-specific gene expression. Under conditions that facilitate muscle cell differentiation, the ID protein levels fall, allowing E12 and/or E47 to form heterodimers with Myo D and myogenin, which in turn activate myogenic differentiation. It has been shown that expression of each of the ID proteins is strongly dependent on growth factor activation and that reduction of ID mRNA levels by antisense oligonucleotides leads to a delayed reentry of arrested cells into the cell cycle following growth factor stimulation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.