Thermo Fisher Scientific ID3 Monoclonal Antibody (2B11)
ID3 단백질을 인식하는 Mouse monoclonal antibody (clone 2B11). Western blot, ICC/IF, IP, ChIP 등 다양한 응용에 적합. Human 및 Mouse 반응성. Protein G 정제, PBS 용액 형태로 제공. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–5 µg/mL | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | - |
| Immunoprecipitation (IP) | 1–5 µg/mL | View 1 publication |
| ChIP assay (ChIP) | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2B11 |
| Immunogen | The full-length (amino acids 1–119) mouse Id3 gene expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2.45 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2295760 |
Product Specific Information
Recommended positive controls: Id3-transfected 293T, Id3-transfected Sol8 muscle cells, and recombinant fusion protein.
Target Information
Members of the Id family of basic helix-loop-helix (bHLH) proteins include ID1, ID2, ID3, and ID4. They are ubiquitously expressed and form heterodimers with class A and B HLH proteins. Due to the absence of the basic region, these heterodimers cannot bind DNA, thus negatively regulating DNA binding of bHLH proteins.
ID1 inhibits DNA binding of E12 and Myo D, thereby suppressing muscle-specific gene expression. During muscle cell differentiation, ID protein levels decrease, allowing E12/E47 to interact with Myo D and myogenin to activate myogenic differentiation. Expression of ID proteins is growth factor-dependent, and reduction of ID mRNA delays cell cycle reentry after growth factor stimulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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