Thermo Fisher Scientific Dopamine beta Hydroxylase Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000

-

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

-

Immunocytochemistry (ICC/IF)

1:2,500

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bovine, Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Sheep / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues S(43) E P P E S P F P Y H I P L D P E G T L(62) of rat DBH. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Dopamine beta Hydroxylase,uniProtId:Q05754-1,ncbiNodeId:10114,antigenRange:44-62,antigenLength:620,antigenImageFileName:PA3-925_Dopamine_beta_Hydroxylase_Q05754-1_Sheep.svg,antigenImageFileNamePDP:PA3-925_Dopamine_beta_Hydroxylase_Q05754-1_Sheep_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

whole serum

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2089215

Product Specific Information

PA3-925 detects human, bovine, rat and mouse DBH. This antibody is not effective for immunohistochemistry in human brain.

PA3-925 has been successfully used in Western blot, immunohistochemistry and immunofluorescence procedures. By Western blot, this antibody detects an ~72 kDa protein representing DBH from rat neurons. In immunohistochemistry and immunofluorescence procedures, it specifically stains chromaffin cells of the adrenal medulla, and DBH containing cells of the sympathetic nervous system.

The PA3-925 immunogen is a synthetic peptide corresponding to residues S(43) E P P E S P F P Y H I P L D P E G T L(62) of rat DBH. This sequence is highly conserved (>85% homology) in human, bovine and mouse.

Target Information

The protein encoded by this gene is an oxidoreductase belonging to the copper type II, ascorbate-dependent monooxygenase family. It is present in the synaptic vesicles of postganglionic sympathetic neurons and converts dopamine to norepinephrine. It exists in both soluble and membrane-bound forms, depending on the absence or presence, respectively, of a signal peptide.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.