Thermo Fisher Scientific Dopamine beta Hydroxylase Polyclonal Antibody
도파민을 노르에피네프린으로 전환하는 DBH 단백질을 검출하는 폴리클로날 항체. Western blot, IHC, ICC/IF에 사용 가능. 사람, 소, 쥐, 랫트 반응성. 액상 형태로 보관하며 -20°C에서 저장. 연구용 전용 제품.
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Thermo Fisher Scientific Dopamine beta Hydroxylase Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:5,000 | - |
| Immunohistochemistry (IHC) | - | View 2 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent | - |
| Immunocytochemistry (ICC/IF) | 1:2,500 | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bovine, Human, Mouse, Rat |
| Published Species | Human, Mouse |
| Host/Isotype | Sheep / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to residues S(43) E P P E S P F P Y H I P L D P E G T L(62) of rat DBH |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not Determined |
| Storage Buffer | Whole serum |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2089215 |
Product Specific Information
PA3-925 detects human, bovine, rat, and mouse DBH. This antibody is not effective for immunohistochemistry in human brain.
It has been successfully used in Western blot, immunohistochemistry, and immunofluorescence procedures.
By Western blot, this antibody detects an ~72 kDa protein representing DBH from rat neurons.
In immunohistochemistry and immunofluorescence procedures, it specifically stains chromaffin cells of the adrenal medulla and DBH-containing cells of the sympathetic nervous system.
The PA3-925 immunogen is a synthetic peptide corresponding to residues S(43)–L(62) of rat DBH, which is highly conserved (>85% homology) in human, bovine, and mouse.
Target Information
The protein encoded by this gene is an oxidoreductase belonging to the copper type II, ascorbate-dependent monooxygenase family.
It is present in the synaptic vesicles of postganglionic sympathetic neurons and converts dopamine to norepinephrine.
It exists in both soluble and membrane-bound forms, depending on the absence or presence of a signal peptide.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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