Thermo Fisher Scientific E2F1 Polyclonal Antibody
E2F1 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, ELISA, Immunoprecipitation에 사용 가능. Human, Mouse, Rat 시료에 반응하며, 고순도 친화 크로마토그래피 정제. 세포주기 조절 및 종양억제 연구용에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1:50–1:250 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide within amino acid region 380–430 on E2F1 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5–1.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer, pH 7.4–7.8, with 30% glycerol, 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | −20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of the cell cycle and the action of tumor suppressor proteins, and is also a target of the transforming proteins of small DNA tumor viruses.
E2F proteins contain several evolutionarily conserved domains, including:
- DNA binding domain
- Dimerization domain (interacts with DP transcription factors)
- Transactivation domain enriched in acidic amino acids
- Tumor suppressor protein association domain (within the transactivation domain)
E2F1, along with E2F2 and E2F3, also contains a cyclin binding domain and binds preferentially to retinoblastoma protein (pRB) in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent or -independent apoptosis.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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