Thermo Fisher Scientific E2F1 Polyclonal Antibody, Biotin
E2F1 단백질을 인식하는 Biotin 결합 Rabbit Polyclonal Antibody로, Human, Mouse, Rat 시료에 반응합니다. Western Blot, ELISA, Immunoprecipitation 등 다양한 응용에 사용 가능하며, 고순도 Affinity chromatography로 정제되었습니다. 연구용으로만 사용됩니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1:50–1:250 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide within amino acid region 380–430 on E2F1 protein |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5–1.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer, pH 7.4–7.8, with 30% glycerol, 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | –20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of the cell cycle and the action of tumor suppressor proteins. It is also a target of transforming proteins from small DNA tumor viruses. E2F proteins contain several conserved domains, including a DNA-binding domain, a dimerization domain for interaction with DP proteins, a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain embedded within the transactivation domain. E2F1, along with E2F2 and E2F3, possesses an additional cyclin-binding domain and binds preferentially to retinoblastoma protein pRB in a cell-cycle-dependent manner. It can mediate both cell proliferation and p53-dependent or independent apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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