Thermo Fisher Scientific FIX & PERM Cell Permeabilization Kit, 50 tests

Invitrogen™ FIX & PERM™ Cell Permeabilization Kit

The FIX & PERM™ Cell Permeabilization Kit is used in flow cytometry for fixing and permeabilizing cells in suspension, allowing access of antibodies to intracellular targets without sacrificing scatter characteristics.

주요 특징

• Flexible — compatible with multiple sample types and cellular antigens
• Robust — scatter pattern of cells unaffected
• Efficient workflow — simultaneous detection of intracellular and surface markers

제품 설명

The FIX & PERM™ Cell Permeabilization Kit is suitable for various biological samples and has been validated for use with cell lines, whole blood, and bone marrow.
This kit enables accurate identification of previously undetectable intracellular markers.
Unlike other fixation and permeabilization reagents, the FIX & PERM™ protocol preserves morphological scatter characteristics of the cells.

The FIX & PERM™ formula is specifically designed to reduce background staining and allow simultaneous addition of fluorochrome-labeled antibodies during the permeabilization step.

작동 원리

The FIX & PERM™ Cell Permeabilization Kit contains two reagents:

1. Fixation Medium (Reagent A)
2. Permeabilization Medium (Reagent B)

Cells are incubated for 15 minutes at room temperature with the fixation medium, washed, and then incubated with the permeabilization medium and fluorochrome-labeled antibody.

제품 구성 및 옵션

The FIX & PERM™ Cell Permeabilization Kit is available in two sizes: 50 tests and 200 tests.
Reagent A and Reagent B are also available separately in bulk size (1000 tests).

사양