Thermo Fisher Scientific PPM1B Monoclonal Antibody (OTI3E8), TrueMAB
PPM1B 단백질을 인식하는 Mouse Monoclonal Antibody로, Western blot과 IHC(Paraffin)에서 검증됨. HEK293 세포에서 발현된 full-length human recombinant protein을 면역원으로 사용. 동결건조 형태로 제공되며, 재구성 후 약 1 mg/mL 농도로 사용 가능. 연구용으로 세포 스트레스 및 cell cycle 조절 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3E8 |
| Immunogen | Full-length human recombinant protein of human PPM1B produced in HEK293 cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882) to ensure carrier-free conditions.
Target Information
The PPM1B gene encodes a member of the PP2C family of serine/threonine protein phosphatases, which act as negative regulators of cell stress response pathways.
This phosphatase dephosphorylates cyclin-dependent kinases (CDKs), implicating it in cell cycle control. Overexpression can induce cell growth arrest or apoptosis.
Alternative splicing produces multiple transcript variants encoding different isoforms. Some additional variants are described but may not represent full-length sequences.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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