Thermo Fisher Scientific CRY2 Monoclonal Antibody (OTI1H5), TrueMAB
Human CRY2 단백질을 인식하는 mouse monoclonal antibody로 Western blot, IHC, ICC, Flow cytometry에 적합. Lyophilized 형태로 제공되며, 높은 순도의 affinity chromatography 정제. Circadian rhythm 연구용으로 적합.
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Thermo Fisher Scientific CRY2 Monoclonal Antibody (OTI1H5), TrueMAB
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1H5 |
| Immunogen | Full length human recombinant CRY2 protein produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
Cryptochrome 1 (CRY1) and Cryptochrome 2 (CRY2) are flavoproteins involved in circadian rhythm regulation. They interact with CLOCK, BMAL1, PER1, PER2, and TIM genes, functioning as light-independent components of the circadian clock.
Mutations in Cry1 or Cry2 affect locomotor activity rhythms in mice, demonstrating their essential role in maintaining circadian synchronization. A balanced expression of Cry1 and Cry2 is crucial for proper clock function; double-mutant mice exhibit arrhythmicity in complete darkness.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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