Thermo Fisher Scientific CD41a Monoclonal Antibody (HIP8), NovaFluor Yellow 590, eBioscience

Applications

• Flow Cytometry (Flow): 5 µL (0.6 µg)/test

Product Specifications

Additional Formats

• Unconjugated
• APC
• APC-eFluor 780
• eFluor 450
• FITC
• NovaFluor Blue 510
• NovaFluor Blue 585
• NovaFluor Blue 660-120S
• NovaFluor Yellow 660
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Custom conjugation available

Product Specific Information

The HIP8 monoclonal antibody reacts with human CD41 (integrin αIIb, platelet GPIIb). CD41 associates with integrin β3 (CD61) and is expressed by megakaryocytes and platelets. The CD41/CD61 complex acts as a receptor for fibronectin, fibrinogen, von Willebrand factor, vitronectin, and thrombospondin, mediating platelet aggregation. HIP8 blocks platelet aggregation.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated for flow cytometry using normal human peripheral blood cells. Recommended use: 5 µL (0.6 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains with NovaFluor dyes.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining.
• Add 5 µL of CellBlox Plus per sample before combining antibodies with cells.
• For single-color controls, use 5 µL per 100 µL cell sample (10³–10⁸ cells).

NovaFluor conjugates use Phiton™ technology, employing novel nucleic acid dye structures engineered for optimized fluorescence performance with minimal spillover.

Excitation: 552 nm
Emission: 592 nm
Laser: 561 nm (Yellow)

Target Information

CD41 (platelet glycoprotein IIb, ITGA2B) consists of two subunits (120 kDa α and 23 kDa β) that interact with CD61 in the presence of calcium to form a functional adhesive receptor. CD41 mediates platelet aggregation and binds von Willebrand factor, fibrinogen, fibronectin, and vitronectin upon vascular injury. Expressed mainly on megakaryocyte-platelet lineage, CD41 is also present during early hematopoietic differentiation.

Associated Diseases:

• Glansmann Thrombasthenia
• Platelet type-16 bleeding disorder

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

제품 이미지