Thermo Fisher Scientific LCK Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 0.1–1.0 µg/mL

Product Specifications

Target Information

LCK is a member of the Src-family tyrosine kinases, essential for signaling through the T-cell receptor (TCR) complex. Upon TCR triggering, LCK phosphorylates ITAM motifs in the zeta subunits, creating binding sites for the SH2 domains of ZAP70, which is subsequently phosphorylated and activated by LCK to propagate downstream signaling via adaptor LAT.

Most LCK localizes to the plasma membrane, with a fraction associated with the Golgi apparatus that may contribute to Raf activation under weak TCR stimulation. LCK contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains mediating interactions with phosphotyrosine-containing and proline-rich motifs. LCK also regulates apoptosis induced by various stimuli, excluding death receptor pathways.

Diseases associated with LCK include Immunodeficiency 22 and CD45 deficiency. Alternative splice variants encoding different isoforms have been identified.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.