Thermo Fisher Scientific Phospho-LCK (Tyr192) Polyclonal Antibody

Thermo Fisher Scientific Phospho-LCK (Tyr192) Polyclonal Antibody

Applications and Tested Dilution

• Western Blot (WB): 1:1,000

Product Specifications

Target Information

LCK is a member of the Src-family tyrosine kinases, essential for signaling through the T-cell receptor (TCR) complex. Upon TCR triggering, LCK phosphorylates ITAM motifs in the zeta subunits, establishing binding sites for the SH2 domains of ZAP70, which is also phosphorylated by LCK to generate signaling platforms via LAT phosphorylation.
Most LCK is localized to the plasma membrane, with a fraction associated with the Golgi apparatus that may contribute to Raf activation under weak TCR stimulation.
LCK contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2/SH3 domains for protein-protein interactions. It is also involved in apoptosis regulation induced by various stimuli, excluding death receptors.
Diseases associated with LCK include Immunodeficiency 22 and CD45 deficiency. Alternative splice variants encoding different isoforms have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.