
Thermo Fisher Scientific Goat anti-Human IgM (Heavy chain) Cross-Adsorbed Secondary Antibody, HRP
HRP로 결합된 염소 유래 항-인간 IgM(Heavy chain) 2차 항체로, Western blot, IHC, ELISA에 사용 가능. Human IgA 및 IgG에 대한 교차 반응 최소화. 친화 크로마토그래피로 정제된 고순도 폴리클로날 항체. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Dilution Range |
|---|---|
| Western Blot (WB) | 1:500–1:20,000 |
| Immunohistochemistry (IHC) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Human Mu immunoglobulin |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.1% ProClin 150 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2535618 |
| Target | IgM (Heavy chain) |
| Cross Adsorption | Against human IgA and IgG |
| Antibody Form | Whole Antibody |
Product Specific Information
- Rehydrate with 1.1 mL of deionized water and let stand 30 minutes at room temperature to dissolve.
- Centrifuge to remove any particulates.
- Prepare fresh working dilution daily.
- Store lyophilized material at 2–8°C.
- For long-term storage after reconstitution, dilute with 50% glycerol and store at -20°C as a liquid.
- Based on immunoelectrophoresis, no reactivity is observed to non-immunoglobulin human serum proteins, light chains on all human immunoglobulins, human IgA or IgG.
Target Information
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins. They are useful for detection, sorting, or purification of the specified target.
Secondary antibodies offer increased versatility and sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
Typically, these antibodies are generated by immunizing the host animal with pooled human immunoglobulins and further purified or modified (e.g., immunoaffinity chromatography, fragmentation, label conjugation) to produce highly specific reagents.
Available Conjugate Formats
- Alexa Fluor 488
- Alexa Fluor 594
- Alexa Fluor 647
- Alkaline Phosphatase (AP)
- Biotin
- FITC
- Custom conjugation available upon request
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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