
Thermo Fisher Scientific Goat anti-Human IgM (Heavy chain) Cross-Adsorbed Secondary Antibody, FITC
인간 IgM(Heavy chain)에 특이적인 염소 유래 2차 항체로, FITC 형광 표지. ICC/IF 및 유세포분석에 사용 가능. 교차흡착 처리로 비특이적 결합 최소화. 고순도 친화 크로마토그래피 정제. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunocytochemistry (ICC/IF) | 1:500–1:20,000 |
| Flow Cytometry (Flow) | 1:500–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Human Mu immunoglobulin |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.8, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C avoiding freeze/thaw cycles. Store in the dark. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2535619 |
| Target | IgM (Heavy chain) |
| Cross Adsorption | Against human IgA and IgG |
| Antibody Form | Whole Antibody |
Additional Formats
- Alexa Fluor 488
- Alexa Fluor 594
- Alexa Fluor 647
- AP
- Biotin
- HRP
- Custom conjugation available upon request
Target Information
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins. These antibodies are useful for detection, sorting, or purification of the specified target. Secondary antibodies offer increased versatility, enabling the use of multiple detection systems such as HRP, AP, or fluorescence. They also enhance sensitivity through signal amplification since multiple secondary antibodies can bind to a single primary antibody. Typically, secondary antibodies are generated by immunizing the host animal with pooled immunoglobulins from the target species and can be further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to produce highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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